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E’ l’ansia che fa invecchiare le donne
Luglio 16th, 2012 by admin

macch-neraManteniamo la calma, è l’ansia che fa invecchiare le donne!

Fra chi ne soffre e chi ne è immune è come se ci fosse una differenza di 6 anni. Ma la meditazione protegge invece dall’invecchiamento, vedi http://www.sangye.it/dalailamanews/?p=3483#more-3483 allungando le stesse strutture del DNA , i telomeri (quindi allungando la vita) danneggiati invece dall’ansia. Essere calme e’ una scelta che paga per le donne, perché l’ansia oltre a molte altre problematiche porta con se’ anche quella di un invecchiamento più rapido. E’ quanto dimostra una ricerca pubblicata sulla versione online della rivista PlosOne, condotta dal Brigham and Women’s Hospital di Boston. Secondo i ricercatori le donne affette dalla forma più comune di ansia, cioè quella fobica, avrebbero i telomeri più corti. I telomeri sono la parte terminale del Dna, proteggono il materiale genetico da danneggiamenti e il loro progressivo accorciamento e’ associato all’invecchiamento cellulare: averli più corti e’ sintomo del fatto che l’invecchiamento cellulare avviene prima. Per arrivare a questi risultati sono stati esaminati i campioni di sangue di 5mila donne di eta’ compresa tra i 42 e i 69 anni, a cui sono stati anche somministrati dei questionari relativi ad eventuali forme di ansia fobica. Ne e’ emerso che ad alti livelli di ansia corrispondevano telomeri più corti: in particolare la differenza tra una donna ansiosa e una non ansiosa in termini di invecchiamento cellulare e’ stata quantificata come pari a sei anni. “Molte persone si chiedono se e come sia possibile che lo stress possa farci invecchiare più rapidamente – spiega la dottoressa Olivia Okereke, che ha condotto lo studio – questo studio mostra una notevole connessione tra una comune forma di stress psicologico, l’ansia fobica, e un meccanismo plausibile di invecchiamento precoce”. (ANSA).

High Phobic Anxiety Linked to Relative Telomere Length

Older women who have phobic anxiety have shorter relative telomere lengths, according to a study published online July 11 in PLoS One.

    FRIDAY, July 13 (HealthDay News) — Older women who have phobic anxiety have shorter relative telomere lengths, according to a study published online July 11 in PLoS One. Olivia I. Okereke, M.D., from Brigham and Women’s Hospital in Boston, and colleagues measured relative telomere lengths in peripheral blood leukocytes from 5,243 nurses (42 to 69 years old) who had completed the Crown-Crisp index, which measures symptoms of phobic anxiety. The researchers found that women with higher phobic anxiety generally had shorter relative telomere lengths, even after adjusting for possible confounders, including smoking and physical activity. For the most phobic women (Crown-Crisp index of six or greater) the difference in relative telomere length was comparable to that of a woman six years older. The association was stronger in women who were overweight, had never smoked, and had been born to fathers who were 40 years or older. “In summary, high phobic anxiety may be associated with shorter leukocyte relative telomere lengths in middle-aged and older women,” Okereke and colleagues conclude. “Identification of these novel associations invites further investigation in large-scale prospective studies, with detailed ascertainment of anxiety and other mental health variables and repeated measures of telomere length.”

Abstract (http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0040516#abstract0) Full Text (http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0040516 )

High Phobic Anxiety Is Related to Lower Leukocyte Telomere Length in Women

Olivia I. Okereke1,2,5*, Jennifer Prescott1,2,3, Jason Y. Y. Wong1,2,3, Jiali Han1,2,3,4, Kathryn M. Rexrode6, Immaculata De Vivo1,2,3

Channing Laboratory, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston, Massachusetts, United States of America, 2 Department of Epidemiology, Harvard School of Public Health, Boston, Massachusetts, United States of America, 3 Program in Molecular and Genetic Epidemiology, Harvard School of Public Health, Boston, Massachusetts, United States of America, 4 Clinical Research Program, Department of Dermatology, Brigham and Women’s Hospital and Harvard Medical School, Boston, Massachusetts, United States of America, 5 Department of Psychiatry, Brigham and Women’s Hospital and Harvard Medical School, Boston, Massachusetts, United States of America, 6 Division of Preventive Medicine, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston, Massachusetts, United States of America

Background

Chronic psychological distress has been linked to shorter telomeres, an indication of accelerated aging. Yet, little is known about relations of anxiety to telomeres. We examined whether a typically chronic form of anxiety – phobic anxiety – is related to telomere length.

Methodology/Principal Findings

Relative telomere lengths (RTLs) in peripheral blood leukocytes were measured by quantitative real-time polymerase chain reaction among 5,243 women (aged 42–69 years) who: were participants in the Nurses’ Health Study; were controls in prior case-control studies of telomeres and disease, or randomly selected healthy participants in a cognitive function sub-study; had completed the Crown-Crisp phobic index proximal to blood collection. Adjusted least-squares mean RTLs (z-scores) were calculated across phobic categories. Higher phobic anxiety was generally associated with lower RTLs (age-adjusted p-trend = 0.09); this association was similar after adjustment for confounders – paternal age-at-birth, smoking, body mass index (BMI) and physical activity (p-trend = 0.15). Notably, a threshold was identified. Among women with Crown-Crisp<6 points, the multivariable-adjusted least-squares mean RTL z-score = 0.02 standard units; however, among the most phobic women (Crown-Crisp≥6), the multivariable-adjusted least-squares mean RTL z-score = −0.09 standard units (mean difference = −0.10 standard units; p = 0.02). The magnitude of this difference was comparable to that for women 6 years apart in age. Finally, effect modification by BMI, smoking and paternal age was observed: associations were stronger among highly phobic women with BMI≥25 kg/m2, without smoking history, or born to fathers aged ≥40 years.

Conclusions/Significance

In this large, cross-sectional study high phobic anxiety was associated with shorter telomeres. These results point toward prospective investigations relating anxiety to telomere length change.

Citation: Okereke OI, Prescott J, Wong JYY, Han J, Rexrode KM, et al. (2012) High Phobic Anxiety Is Related to Lower Leukocyte Telomere Length in Women. PLoS ONE 7(7): e40516. doi:10.1371/journal.pone.0040516

Editor: Xiang Yang Zhang, Baylor College of Medicine, United States of America

Received: February 29, 2012; Accepted: June 12, 2012; Published: July 11, 2012

Copyright: © 2012 Okereke et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Funding: This work was supported by National Institutes of Health (NIH) grants R01 CA082838, P01 CA87969, R01 CA49449, R01 HL034594, R01 HL088521, R01 CA065725, R03 CA132190, R03 CA139586 and K07 CA140790. Dr. Okereke was supported by the Harvard Medical School Eleanor and Miles Shore Fellowship and NIH Career Development Award K08 AG029813. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Competing interests: The authors have declared that no competing interests exist.

* E-mail: olivia.okereke@channing.harvard.edu

Introduction Top

Telomeres are repetitive DNA sequences at the ends of eukaryotic chromosomes that undergo attrition each time a somatic cell divides. Factors that accelerate attrition include oxidative stress and inflammation [1], [2]. Consequently, average telomere length (TL) reflects cumulative damage from these exposures, and is a potential indicator of biological aging [3]. Furthermore, by protecting chromosomal ends, telomeres maintain genomic stability [4], [5], [6]; critically short telomeres lead to DNA damage (e.g., end-to-end fusion, atypical recombination or rearrangement) implicated in development of several age-related diseases [7]. For example, epidemiological studies have reported associations between shorter TLs and increased risks of cancers [8], [9] and cancer mortality [10], cardiovascular disease [7], cognitive decline and dementia [11], [12]. Because telomere shortening may underlie many adverse health outcomes in aging, it is important to identify addressable risk factors.

An emerging literature implicates psychological distress and mood disorders, both highly prevalent in women, as potential paths toward accelerated aging [13], [14], [15], [16]. Prior work has indentified relations of depression to higher levels of inflammatory mediators and oxidative stress [17]. Although less is known about its relations to these mechanistic paths, anxiety could also be a risk factor for morbidity or mortality in aging. In a previous investigation in the Nurses’ Health Study (NHS), phobic anxiety was significantly related to higher levels of inflammatory markers [18] and a elevated risk of sudden cardiac death and fatal coronary disease [19]. Importantly, phobic anxiety is treatable; thus, any potential impacts on telomere shortening may be amenable to prevention through early identification and treatment. However, there have been few prior investigations of anxiety and telomere shortening.

Thus, we conducted an examination of the relation of phobic anxiety to peripheral blood leukocyte TLs (LTLs) in 5,243 participants of the Nurses’ Health Study, who ranged from age 42–69 years (mean = 59) when they provided self-reports of phobic symptoms and blood samples. As phobic anxiety tends to be chronic – median age at onset is 11 years [20], and course tends to be highly persistent without treatment [21], [22], [23] – we hypothesized strong inverse associations between phobic anxiety and LTLs measured at mid- and later-life.

Methods

Study population

The Nurses’ Health Study (NHS) included 121,700 U.S., female registered nurses, aged 30 to 55 years at the study’s inception in 1976. Since then, participants have completed biennial mailed questionnaires updating information on numerous lifestyle factors and health outcomes; total follow-up exceeds 90%. Details regarding the NHS and validation of various health exposures and endpoints have been published previously [24], [25], [26]. During the 1988–1990 questionnaire cycle, participants were asked to complete the Crown-Crisp Index (CCI) of phobic anxiety [27], [28]. From 1989 to 1990, 32,826 NHS participants provided blood samples; details of the blood collection and archival methods have been described previously [29], [30]. The protocols for the NHS, the NHS blood collection, and the current study were approved by the Institutional Review Board of Brigham and Women’s Hospital, Boston, MA, USA. Blood donors gave written informed consent to having their samples used for research purposes. In addition, all data was analyzed anonymously.

To determine the final study population, we considered participants from nested case-control studies of pre-diagnostic LTLs and incident cancers and cardiovascular disease (CVD), and from a study of cognitive function (n = 9,190) [12], [31], [32], [33]. Although blood collections occurred among both cases and controls prior to onset of the diseases of interest, we utilized the most conservative approach and minimized possible bias by including only controls and the random sample of cognitive study participants (n = 5,415). We excluded women with insufficient CCI data (i.e., missing >2 items) (n = 146) or missing LTL values (n = 26). Thus, the sample for analysis included 5,243 women.

Assessment of phobic anxiety: the Crown-Crisp index

The CCI measures symptoms of phobic anxiety; it has been validated in psychiatric outpatient settings and found to discriminate patients with general anxiety and phobias from healthy controls or those with other conditions (i.e., obsessive-compulsive or depressive) [27], [28]. The CCI primarily covers aspects of “fear” disorders, such as panic and agoraphobia. The index features 8 self-rated questions, and total scores range from 0 to 16 points (higher scores indicate higher anxiety). For those missing data on 1 or 2 items, we chose the more conservative approach of basing the CCI sum score on only the answered items [34] (i.e., rather than imputing scores using the mean [19], [35]). CCI scores are not normally distributed, and in keeping with other work [36], [37] we categorized CCI scores into 5 groups: 0 or 1 point (reference group); 2 points; 3 points; 4 or 5 points; and ≥6 points (highest phobic anxiety group; the range of CCI scores in this fifth group = 6–16 points). Of note, the CCI is reliable in the NHS cohort: the Cronbach coefficient alpha for scores in 1988 was 0.62 – comparable to the coefficient of 0.69 originally reported by Crown and Crisp among diagnosed patients and controls [28]; item-total correlations were also in the desirable range [38] (0.26–0.43). Furthermore, identical items were featured on the 2004 NHS questionnaire; the intraclass correlation coefficient (95% confidence interval [CI]) for the pair of CCI scores in 1988 and 2004 was 0.61 (0.61–0.62), and the Fleiss-Cohen-weighted kappa (95% CI) for consistency of the 5 phobic categories was 0.56 (0.55–0.57) – an indication that these measures can reliably represent long-term phobic anxiety levels.

Measurement of leukocyte telomere length

Genomic DNA was extracted from peripheral blood leukocytes using the QiAmp (Qiagen) 96-spin blood protocol. Our outcome measure was relative LTL (RTL) using quantitative real-time polymerase chain reaction (Q-PCR) [8]. Average RTL was calculated as the exponentiated ratio of Telomere repeat copy number to Single gene (36B4) copy number (T/S) corrected for a reference sample [39]. Laboratory technicians masked to participant characteristics assayed each sample in triplicate. Quality control samples were interspersed on each plate to assess variability. In all nested case-control studies, coefficients of variation (CVs) for the telomere and single gene assay were <4%, and CVs for the exponentiated T/S ratio were <17%. Although this assay provides a relative measurement of telomere length, T/S ratios correlate well with absolute telomere lengths determined by Southern blot (r = 0.82, p<0.0001) [39]. As expected, significant correlations have been found in the NHS cohort between shorter telomeres and key predictors: age (Spearman partial rank correlation coefficient [rs] = −0.09, p<0.0001), smoking pack-years (rs = −0.04, p = 0.0005) and body mass index (BMI) (rs = −0.04, p = 0.0008) [40].

Assessment of covariates

In addition to age-at-blood collection, we considered number of variables potentially associated with phobic anxiety and telomere length. Using the biennial questionnaires completed proximal (1988–1990) to blood collection and a supplemental questionnaire administered at blood collection, we ascertained factors that have been related to TLs in the NHS and elsewhere: BMI (kg/m2); physical activity (total metabolic equivalent hours of activity per week [MET-hrs/wk]) [40]; cigarette smoking (pack-years) [8], [41], [42]; and paternal age-at-participant’s birth (15–24, 25–29, 30–34, 35–39, ≥40 years) [43]. Also, we obtained information on numerous health, social and lifestyle factors: postmenopausal hormone use, with duration (never or pre-menopausal; past; current <5 years or ≥5 years); oral contraceptive use (ever/never); anthropometric measures (e.g., waist-hip ratio, waist circumference); education (associate’s, bachelor’s, or master’s/doctoral degree) [44]; race/ethnicity; work status (e.g., employed outside home, homemaker, retired, etc.) [45]; alcohol intake (grams/day); daily multivitamin use [46]; medication use, including benzodiazepines (BZDs) (N.B.: regular diazepam use was reported as yes/no in 1980 and 1982; data on regular antidepressant use was not available until 1996); and presence/absence of chronic comorbidities (e.g., diabetes, hypertension). Finally, using a validated semi-quantitative food frequency questionnaire [47] administered in 1990, we assessed dietary factors (e.g., calories, macronutrients, n-6 and n-3 fatty acids [48], caffeine intake, vitamin intake [46], antioxidant fruit and vegetable consumption).

Statistical analyses

The distribution of RTLs varied across batch sets from each nested case-control study and the cognitive study. Thus, we computed RTL z-scores within each batch, after calculating the natural logarithm to improve normality.

To examine potential for confounding, we first examined univariate associations of the factors described above with phobic categories and RTL z-scores; variables with significant or borderline relations to phobic anxiety or RTL were addressed in preliminary model-building steps. We assessed for threshold associations using the 5 phobic categories and for linear trends by treating phobic category as an ordinal predictor.

To examine the association between phobic anxiety and RTLs, we estimated adjusted least-squares mean RTL z-scores using three generalized linear models. In the first model, we adjusted for age-at-blood draw in years. In the second model, we additionally adjusted for the largest potential contributors to confounding: BMI, physical activity, pack-years of smoking and paternal age. Sequentially, we considered other covariates (described above) as potential confounders, based on prior literature, putative associations to oxidative stress, or preliminary univariate analyses. As there were no changes in the estimates with inclusion of these covariates, they were omitted from the final multivariate model. Thus, the third model was the primary model and it included: participant age, paternal age, smoking, BMI and physical activity; indicator variables were created where covariates had missing values.

We conducted key secondary analyses to address sensitivity and to further explore phobic-telomere associations. First, we further adjusted the primary model for major conditions that are potential confounders and/or intermediates: hypertension, diabetes, dyslipidemia, and CVD. Second, we utilized multiplicative interaction terms to evaluate whether associations differed by participant age (<60/≥60 years), older parental age (mother <35/≥35 years; father <40/≥40 years [49]), BMI (18.5–25 vs. ≥25 kg/m2), smoking (ever/never), physical activity (above/below median), or BZD use (yes/no), as both biology and prior literature support potential modifying roles [50], [51], [52]. Third, as some participants had major comorbidities that could contribute to higher self-reported anxiety symptom levels as well as shorter telomeres (e.g., obstructive airways diseases show strong associations with panic and anxiety) [53] we conducted separate analyses excluding women diagnosed with prevalent CVD [54], diabetes [55], or chronic obstructive airways diseases (asthma, chronic bronchitis or emphysema) [26], [56] prior to or concurrent with blood collection (total n-excluded = 594). Finally, we conducted a separate analysis to explore relations of individual CCI items to RTLs, adjusting for age. All statistical analyses were performed using SAS version 9.2 (SAS Institute Inc, Cary, NC). Hypothesis testing was 2-sided, with α = 0.05.

Results Top

Table 1 shows sample characteristics at blood draw, across phobic anxiety levels. Women in the highest phobic category were generally less healthy than those in the lowest category: e.g., higher phobic anxiety was associated with lower physical activity and educational attainment, and with higher BMI, smoking pack-years, and prevalence of hypertension, CVD, obstructive airways diseases, diabetes, and dyslipidemia. Women with higher phobic symptoms were less likely to use daily multivitamins or to have used oral contraceptives, and more likely to have used BZDs. Regarding diet, slightly higher daily calories, lower energy from carbohydrates, protein and omega-3 fatty acids, and lower vitamin D intake were observed among more phobic women; there were no other consistent differences by phobic anxiety in intake of other dietary factors.

thumbnailTable 1. Characteristics by phobic anxiety categories (lowest to highest) at blood draw (n = 5,243)*.

doi:10.1371/journal.pone.0040516.t001

Higher phobic anxiety was associated with lower age-adjusted mean RTL z-scores (p-trend = 0.09); estimates were similar after adjustment for paternal age-at-birth, smoking, BMI and physical activity (p-trend = 0.15) (Table 2). However, the phobic anxiety-RTL association was characterized by a threshold effect (Table 3). Although women with CCI<6 had adjusted mean RTL z-scores of 0.02 standard units, those with CCI≥6 had mean RTLs of −0.09 standard units (mean difference = −0.10 standard units; p = 0.02 for difference in means). When compared to the adjusted mean RTL z-score for one year of age (−0.015 standard units, p<0.0001), the magnitude of this difference was equivalent to that observed for women 6 years apart in age.

thumbnailTable 2. Least-squares mean leukocyte telomere length z-scores (standard errors), by phobic anxiety categories.

doi:10.1371/journal.pone.0040516.t002

thumbnailTable 3. Least-squares mean leukocyte telomere length z-scores (standard errors), by high phobic anxiety.

doi:10.1371/journal.pone.0040516.t003

Further adjustment of the primary multivariate model for major medical conditions had no influence on results (Table 2). There were no interactions of high phobic anxiety with participant age, maternal age, physical activity or prior BZD use. However, significant interactions were observed by BMI, smoking and advanced paternal age (APA). Compared with leaner women (BMI<25 kg/m2), associations between high phobic anxiety and lower RTLs were stronger among overweight/obese women (Figure 1). Mean RTLs were lower overall among smokers, but there was no association between high phobic anxiety and lower RTLs; by contrast, the differences in telomere lengths were striking among never-smokers (Figure 2). The mean difference (95% CI) in RTL z-scores comparing never-smoking women with CCI≥6 vs. CCI<6 was −0.25 (−0.38,−0.12) standard units (p<0.001). There was no interaction between BMI and smoking or 3-way interaction of phobic anxiety, BMI and smoking. Finally, a significant phobic anxiety-paternal age interaction was identified; differences in RTLs were more pronounced among highly phobic women born to fathers aged ≥40 years (Figure 3). In the models excluding participants with prevalent CVD, diabetes or obstructive airways diseases, estimates were attenuated. For example, after exclusion of 330 women with CVD or diabetes, the mean difference in RTLs comparing women with CCI≥6 vs. CCI<6 was −0.09 standard units (p = 0.04); with exclusion of an additional 264 participants with obstructive airways diseases, the mean difference was −0.07 standard units (p = 0.12). Significant interactions with BMI, smoking and APA remained after all chronic disease exclusions, but estimates were attenuated: e.g., among never-smokers, the mean difference in RTLs comparing women with CCI≥6 vs. CCI<6 was −0.20 standard units (p = 0.004).

thumbnailFigure 1. Adjusted* least-squares mean leukocyte telomere length z-scores, by phobic anxiety level, stratified by body mass index (<25 kg/m2 or ≥25 kg/m2).

*, Models were adjusted for age in years (continuous), paternal age-at-birth (15–24, 25–29, 30–34, 35–39, ≥40 years), pack-years of smoking (0, >0 to <20, 20 to <40, ≥40), and physical activity (continuous, MET-hrs/wk). Least squares means and 95% confidence intervals are shown. N = 9 excluded due to missing body mass index. , P-value for test of difference in least-squares means.

doi:10.1371/journal.pone.0040516.g001

thumbnailFigure 2. Adjusted* least-squares mean telomere length z-scores, by phobic anxiety level, stratified by smoking status (never or current/past).

*, Models were adjusted for age in years (continuous), paternal age-at-birth (15–24, 25–29, 30–34, 35–39, ≥40 years), body mass index (continuous, kg/m2) and physical activity (continuous, MET-hrs/wk). Least squares means and 95% confidence intervals are shown. N = 20 excluded due to missing smoking status. , P-value for test of difference in least-squares means.

doi:10.1371/journal.pone.0040516.g002

thumbnailFigure 3. Adjusted* least-squares mean telomere length z-scores, by phobic anxiety level, stratified by paternal age (<40 or ≥40 years).

*, Models were adjusted for age in years (continuous), pack-years of smoking (0, >0 to <20, 20 to <40, ≥40), body mass index (continuous, kg/m2) and physical activity (continuous, MET-hrs/wk). Least squares means and 95% confidence intervals are shown. N = 281 excluded due to missing paternal age. , P-value for test of difference in least-squares means.

doi:10.1371/journal.pone.0040516.g003

In exploratory analyses addressing individual CCI items, endorsement of the response option that indicated the highest possible symptom level for a given item was generally associated with lower age-adjusted mean RTLs (Table S1). However, estimates appeared strongest for women with highest endorsement of two items that map to panic and agoraphobia (i.e., compared to women who did not endorse them at all): “feel panicky in crowds” and “uneasy traveling on buses or trains”. Nevertheless, due to the small number of participants who endorsed the highest symptom level for each item, estimates were predictably unstable; only one item, related to agoraphobia (“dislike going out alone”), was statistically significant (p = 0.047) (Table S1).

Discussion Top

In this study of 5,243 women, high phobic anxiety was significantly associated with lower leukocyte telomere lengths. To aid interpretation of findings [40], we can compare the mean difference in RTL z-scores associated with high phobic anxiety to the estimate for women aged one year apart: compared to those with <6 points on the Crown-Crisp, women with ≥6 points had adjusted RTL z-scores equivalent to 6 years of age. In addition, effect modification was identified: associations were stronger among women who were born to older fathers, were heavier or never smoked. Results were somewhat attenuated after exclusion of participants with chronic diseases that may act as intermediates and/or present with higher subjective anxiety and also may be associated with telomere shortening – suggesting that findings may be partly explained by influences of phobic anxiety on risk of development of serious chronic diseases [19]. Finally, exploratory work indicated that endorsements of higher phobic symptom levels on individual CCI items were generally related to lower RTLs; estimates appeared strongest for items reflecting panic and agoraphobia. Overall, this study provides a key addition – phobic anxiety – to an emerging literature that posits mental distress and disorders as risk factors for accelerated aging [13], [14], [16], [57].

This report is consistent with earlier studies involving well-characterized samples of patients with diagnosed psychiatric disorders and controls [14], [58], [59]. However, there have been few larger-scale (n>500) population-based studies that specifically addressed anxiety and telomeres. Kananen et al. [60] measured RTLs in a sample (aged 30–87 years) of 321 participants with anxiety diagnosed via structured clinical interviews and 653 controls. Cases included both those with diagnostic manual criteria-level symptoms and those with core features of anxiety who did not meet full criteria; the majority of cases, however, met full criteria for generalized anxiety disorder (GAD), panic disorder, agoraphobia, and social and other phobia. Although RTLs were similar among cases and controls overall, cases had significantly shorter RTLs than controls in the older half of the sample (48–87 years). In contrast, Surtees et al. [61] did not observe significant differences in RTLs among 4,441 women (aged 41–80 years) with vs. without either 12-month or lifetime GAD, as diagnosed by participant self-assessment forms. Discrepant findings may have resulted from differences in ascertainment methods of diagnoses (self-assessment forms vs. structured interviews by clinical raters) or in the samples (e.g., age, gender, clinical features). For example, the symptoms of disorders under study by Kananen et al. [60] (specifically, panic and phobic disorders) have high overlap with the symptoms captured by the CCI; Surtees and co-workers [61] considered only GAD. Overall, our finding that lower RTLs were observed among those with the highest phobic symptoms – i.e., potentially a “caseness” level – compared to those below that threshold, is consistent with work in clinical samples. Nevertheless, it is not known whether women in the highest phobic symptom category would necessarily meet criteria [62] for diagnosis of an anxiety disorder (particularly, panic or agoraphobia), although this appears plausible.

The findings regarding effect modification of anxiety-telomere relations are novel and require confirmation/replication. Obesity may worsen oxidative stress and inflammation [63], [64], and relations of high phobic anxiety and telomere length may be influenced by body mass or adiposity stores. The findings for smoking were counter-intuitive. CCI scores were higher among ever- vs. never-smokers (Wilcoxon rank sum p = 0.001), smoking may trigger oxidative stress/damage [65], and RTLs were lower overall among smokers in our sample; one might have expected stronger phobic-telomere associations among smokers. Explanations for this finding include chance as well as putative influences of nicotine on key inflammatory mediators [66], [67] – possibly providing protection against telomere shortening for highly phobic smokers that would be absent for equally phobic non-smokers; however, the latter possibility is purely speculative. Finally, findings regarding advanced paternal age were intriguing. APA is associated with longer telomeres [43], but this also allows for more DNA abnormalities to accumulate and greater DNA fragmentation [68]. Intriguingly, APA has recently been related to psychiatric disorders [69], [70] and greater externalizing (vs. internalizing) behaviors in offspring [71]; in our cohort, paternal age ≥40 y vs. <40 y was associated with lower prevalence of high phobic anxiety (12% vs. 9%, χ2(df = 1) = 3.84, p = 0.05). Thus, while explanations remain unclear, one speculative possibility is that high phobic anxiety might exert an exaggerated impact on vulnerable DNA, with resulting faster telomere shortening, among women born to older fathers; alternatively, greater overall variability in TLs among offspring of older fathers may enhance ability to detect phobic anxiety-TL relations.

Although the literature is at an early stage, there is biologic plausibility to support relations of anxiety to shorter telomeres, particularly via oxidative stress and inflammation. For example, in a study [72] of 362 healthy adults, higher tension-anxiety symptom level was correlated with an oxidative DNA damage marker, 8-hydroxydeoxyguanosine. In a previous NHS investigation [18], elevated inflammatory markers (tumor necrosis factor-α receptor II, soluble E-selectin and soluble intercellular adhesion molecule) were observed among diabetic women with the highest phobic anxiety (those with CCI≥6 were grouped in the same category with those with CCI = 4 or 5) [18]. Similarly, higher scores on the Spielberger State-Trait Anxiety Inventory were significantly correlated with elevated C-reactive protein, interleukin-6 and fibrinogen levels in 853 middle-aged adults [73]. Nevertheless, despite high prevalence of anxiety [20], few studies have focused on its relations to oxidative stress and inflammatory biomarkers. Thus, additional investigation is necessary to delineate potential impacts of anxiety on aging via these mechanisms.

Strengths of the current study include: use of a validated phobic anxiety scale; a large, well-characterized sample; and consideration of numerous potential health, lifestyle and socio-demographic confounders. Also, high phobic symptoms may have been present among these participants for decades [23] – highlighting the particular value of relating this exposure to RTLs, which are markers of cumulative aging. Indeed, a connection between early-life exposure to adverse mental health environments and consequences of accelerated aging is an intriguing possibility only recently examined in the literature [61], [74], [75].

Potential limitations should be considered. First, the cross-sectional design precludes establishment of a temporal association between phobic anxiety and telomere length. For example, the possibility of bi-directional links has been raised by recent animal work implicating oxidative stress in development of anxiety [76]. Also, individuals with shorter telomeres may have chronic diseases that contribute to persistence, or even worsening, of phobic symptom levels; however, this possibility appears unlikely to explain results entirely because key findings remained, albeit attenuated, after excluding individuals diagnosed with major comorbidities prior to blood collection. Second, RTL was a single measure, preventing estimation of associations between phobic anxiety and telomere attrition rate. Third, we lacked specific data on anxiety onset, duration and/or treatments and, thus, were unable to incorporate such factors into analyses. Also, we did not concurrently measure depression (such assessments began in NHS in 1992); thus, we cannot exclude confounding by depression (e.g., comorbidity of depressive disorder among persons with anxiety disorders has been estimated at 45% [77]). However, a potentially more likely possibility is that current depression among persons with high phobic anxiety would represent an intermediate variable: phobic conditions typically have early-life onset – decades before median onset of depression [20]; indeed, the “temporal primacy” of anxiety disorders was previously summarized by Kessler [23], [78]. Nevertheless, although it seems unlikely that true confounding by depression would completely explain results, we cannot make conclusions regarding on the role of depression in this analysis. Fourth, as in any observational study, residual confounding is possible. Finally, generalizability is a potential concern; the Nurses were predominantly (95%) of white and European race/ethnicity. Because telomere dynamics may differ among African-Americans and Hispanics [79], [80], the magnitude of associations may not apply to women of other ethnicities or to men. Nevertheless, basic biologic relations between anxiety and telomere lengths are likely to hold in all humans.

In summary, high phobic anxiety may be associated with shorter leukocyte relative telomere lengths in middle-aged and older women. Identification of these novel associations invites further investigation in large-scale prospective studies, with detailed ascertainment of anxiety and other mental health variables and repeated measures of telomere length. Furthermore, because phobic anxiety is usually the temporally primary condition in comorbidity involving other mental disorders (e.g., depression, substance abuse) [23], early intervention not only may mitigate detrimental impacts on aging and but also could avert additional downstream consequences of accelerated telomere shortening due to secondary development of other mental disorders or serious chronic medical conditions.

Supporting Information

Table S1.

Adjusted* least-squares mean telomere length z-scores, according to individual items on the Crown-Crisp Index,* Models were adjusted for age in years (continuous). Total N varies due to missing responses for each item. P-values are from tests of differences in least-squares means, where the lowest symptom level/non-endorsement is the reference category.

Acknowledgments

The authors thank Qun “Carolyn” Guo for assistance with data preparation. We also thank the participants of the Nurses’ Health Study for their years of dedication and commitment.

Author Contributions

Conceived and designed the experiments: OO JP ID. Performed the experiments: OO JP JW JH KR ID. Analyzed the data: OO JP. Contributed reagents/materials/analysis tools: OO JP JW JH KR ID. Wrote the paper: OO. Critical revision of the manuscript for intellectual content: OO JP JW JH KR ID.

References

  1. Saretzki G, Von Zglinicki T (2002) Replicative aging, telomeres, and oxidative stress. Ann N Y Acad Sci 959: 24–29. Find this article online

  2. von Zglinicki T (2002) Oxidative stress shortens telomeres. Trends Biochem Sci 27: 339–344. Find this article online

  3. Aviv A (2004) Telomeres and human aging: facts and fibs. Sci Aging Knowledge Environ 51: pe43. Find this article online

  4. Blasco MA (2005) Telomeres and human disease: ageing, cancer and beyond. Nat Rev Genet 6: 611–622. Find this article online

  5. Blackburn EH (1991) Structure and function of telomeres. Nature 350: 569–573. Find this article online

  6. Blackburn EH (2005) Telomeres and telomerase: their mechanisms of action and the effects of altering their functions. FEBS Lett 579: 859–862. Find this article online

  7. Calado RT, Young NS (2009) Telomere diseases. N Engl J Med 361: 2353–2365. Find this article online

  8. McGrath M, Wong JY, Michaud D, Hunter DJ, De Vivo I (2007) Telomere length, cigarette smoking, and bladder cancer risk in men and women. Cancer Epidemiol Biomarkers Prev 16: 815–819. Find this article online

  9. Wu X, Amos CI, Zhu Y, et al. (2003) Telomere dysfunction: a potential cancer predisposition factor. J Natl Cancer Inst 95: 1211–1218. Find this article online

  10. Willeit P, Willeit J, Mayr A, Weger S, Oberhollenzer F, et al. (2010) Telomere length and risk of incident cancer and cancer mortality. JAMA 304: 69–75. Find this article online

  11. Martin-Ruiz C, Dickinson HO, Keys B, Rowan E, Kenny RA, et al. (2006) Telomere length predicts poststroke mortality, dementia, and cognitive decline. Ann Neurol 60: 174–180. Find this article online

  12. Devore EE, Prescott J, De Vivo I, Grodstein F (2011) Relative telomere length and cognitive decline in the Nurses’ Health Study. Neurosci Lett 492: 15–18. Find this article online

  13. Wolkowitz OM, Epel ES, Reus VI, Mellon SH (2010) Depression gets old fast: do stress and depression accelerate cell aging? Depress Anxiety 27: 327–338; Erratum in: Depress Anxiety. 2010 Jul 2027(2017): 2693. Find this article online

  14. Simon NM, Smoller JW, McNamara KL, Maser RS, Zalta AK, et al. (2006) Telomere shortening and mood disorders: preliminary support for a chronic stress model of accelerated aging. Biol Psychiatry 60: 432–435. Find this article online

  15. Wolkowitz OM, Mellon SH, Epel ES, Lin J, Dhabhar FS, et al. (2011) Leukocyte telomere length in major depression: correlations with chronicity, inflammation and oxidative stress – preliminary findings. PLoS One 6: e17837. Find this article online

  16. Epel ES, Blackburn EH, Lin J, Dhabhar FS, Adler NE, et al. (2004) Accelerated telomere shortening in response to life stress. Proc Natl Acad Sci U S A 101: 17312–17315. Find this article online

  17. Maes M (2008) The cytokine hypothesis of depression: inflammation, oxidative & nitrosative stress (IO&NS) and leaky gut as new targets for adjunctive treatments in depression. Neuro Endocrinol Lett 9: 287–291. Find this article online

  18. Brennan AM, Fargnoli JL, Williams CJ, Li T, Willett W, et al. (2009) Phobic anxiety is associated with higher serum concentrations of adipokines and cytokines in women with diabetes. Diabetes Care 32: 926–931. Find this article online

  19. Albert CM, Chae CU, Rexrode KM, Manson JE, Kawachi I (2005) Phobic anxiety and risk of coronary heart disease and sudden cardiac death among women. Circulation 111: 480–487. Find this article online

  20. Kessler RC, Berglund P, Demler O, Jin R, Merikangas KR, et al. (2005) Lifetime prevalence and age-of-onset distributions of DSM-IV disorders in the National Comorbidity Survey Replication. Arch Gen Psychiatry 62: 593–602. Find this article online

  21. Bruce SE, Yonkers KA, Otto MW, Eisen JL, Weisberg RB, et al. (2005) Influence of psychiatric comorbidity on recovery and recurrence in generalized anxiety disorder, social phobia, and panic disorder: a 12-year prospective study. Am J Psychiatry 162: 1179–1187. Find this article online

  22. Chartier MJ, Hazen AL, Stein MB (1998) Lifetime patterns of social phobia: a retrospective study of the course of social phobia in a nonclinical population. Depress Anxiety 7: 113–121. Find this article online

  23. Kessler RC, Ruscio AM, Shear K, Wittchen HU (2010) Epidemiology of anxiety disorders. Curr Top Behav Neurosci 2: 21–35. Find this article online

  24. Colditz GA, Martin P, Stampfer MJ, Willett WC, Sampson L, et al. (1986) Validation of questionnaire information on risk factors and disease outcomes in a prospective cohort study of women. Am J Epidemiol 123: 894–900. Find this article online

  25. Colditz GA (1995) The Nurses’ Health Study: a cohort of US women followed since 1976. J Am Med Women’s Assoc 50: 40–44. Find this article online

  26. Barr RG, Herbstman J, Speizer FE, Camargo CA Jr (2002) Validation of self-reported chronic obstructive pulmonary disease in a cohort study of nurses. Am J Epidemiol 155: 965–971. Find this article online

  27. Burgess PM, Mazzocco L, Campbell IM (1987) Discriminant validity of the Crown-Crisp experimental index. Br J Psychiatry 60: 61–69. Find this article online

  28. Crown S, Crisp AH (1966) A short clinical diagnostic self-rating scale for psychoneurotic patients. The Middlesex Hospital Questionnaire (M.H.Q.). Br J Psychiatry 112: 917–923. Find this article online

  29. Hankinson SE, Manson JE, Spiegelman D, Willett WC, Longcope C, et al. (1995) Reproducibility of plasma hormone levels in postmenopausal women over a 2–3-year period. Cancer Epidemiol Biomarkers Prev 4: 649–654. Find this article online

  30. Hankinson SE, Willett WC, Manson JE, Hunter DJ, Colditz GA, et al. (1995) Alcohol, height, and adiposity in relation to estrogen and prolactin levels in postmenopausal women. J Natl Cancer Inst 87: 1297–1302. Find this article online

  31. De Vivo I, Prescott J, Wong JY, Kraft P, Hankinson SE, et al. (2009) A prospective study of relative telomere length and postmenopausal breast cancer risk. Cancer Epidemiol Biomarkers Prev 18: 1152–1156. Find this article online

  32. Han J, Qureshi AA, Prescott J, Guo Q, Ye L, et al. (2009) A prospective study of telomere length and the risk of skin cancer. J Invest Dermatol 29: 415–421. Find this article online

  33. Prescott JM, McGrath M, Lee IM, Buring JE, De Vivo I (2010) Telomere length and genetic analyses in population-based studies of endometrial cancer risk. Cancer 116: 4275–4282. Find this article online

  34. Kroenke K, Spitzer RL, Williams JB, Löwe B (2010) The Patient Health Questionnaire Somatic, Anxiety, and Depressive Symptom Scales: a systematic review. Gen Hosp Psychiatry 32: 345–359. Find this article online

  35. Kroenke K, Spitzer RL, Williams JBW, Monahan PO, Löwe B (2007) Anxiety disorders in primary care: prevalence, impairment, comorbidity, and detection. Ann Intern Med 146: 317–325. Find this article online

  36. Haines A, Cooper J, Meade TW (2001) Psychological characteristics and fatal ischaemic heart disease. Heart 85: 385–389. Find this article online

  37. McGrath M, Kawachi I, Ascherio A, Colditz GA, Hunter DJ, et al. (2004) Association between catechol-O-methyltransferase and phobic anxiety. Am J Psychiatry 161: 1703–1705. Find this article online

  38. Streiner EL, Norma GR (1989) Health Measurement Scales: A Practical Guide to their development and Use. Selecting the Items; pp 39–52. Oxford: Oxford University Press.

  39. Cawthon RM (2002) Telomere measurement by quantitative PCR. Nucleic Acids Res 30: e47. Find this article online

  40. Du M, Prescott J, Kraft P, Han J, Giovannucci E, et al. (2012) Physical activity, sedentary behavior, and leukocyte telomere length in women. Am J Epidemiol 175: 414–422. Find this article online

  41. Mirabello L, Huang WY, Wong JY, Chatterjee N, Reding D, et al. (2009) The association between leukocyte telomere length and cigarette smoking, dietary and physical variables, and risk of prostate cancer. Aging Cell 8: 405–413. Find this article online

  42. Valdes AM, Andrew T, Gardner JP, Kimura M, Oelsner E, et al. (2005) Obesity, cigarette smoking, and telomere length in women. Lancet 366: 662–664. Find this article online

  43. Kimura M, Cherkas LF, Kato BS, Demissie S, Hjelmborg JB, et al. (2008) Offspring’s leukocyte telomere length, paternal age, and telomere elongation in sperm. PLoS Genet 4: e37. Find this article online

  44. Steptoe A, Hamer M, Butcher L, Lin J, Brydon L, et al. (2011) Educational attainment but not measures of current socioeconomic circumstances are associated with leukocyte telomere length in healthy older men and women. Brain Behav Immun 25: 1292–1298. Find this article online

  45. Parks CG, DeRoo LA, Miller DB, McCanlies EC, Cawthon RM, et al. (2011) Employment and work schedule are related to telomere length in women. Occup Environ Med 68: 582–589. Find this article online

  46. Xu Q, Parks CG, DeRoo LA, Cawthon RM, Sandler DP, et al. (2009) Multivitamin use and telomere length in women. Am J Clin Nutr 89: 1857–1863. Find this article online

  47. Feskanich D, Rimm EB, Giovannucci EL, Colditz GA, Stampfer MJ, et al. (1993) Reproducibility and validity of food intake measurements from a semiquantitative food frequency questionnaire. J Am Diet Assoc 93: 790–796. Find this article online

  48. Farzaneh-Far R, Lin J, Epel ES, Harris WS, Blackburn EH, et al. (2010) Association of marine omega-3 fatty acid levels with telomeric aging in patients with coronary heart disease. JAMA 303: 250–257. Find this article online

  49. Reproductive Endocrinology and Infertility Committee, Family Physicians Advisory Committee, Maternal-Fetal Medicine Committee, Executive and Council of the Society of Obstetricians, Liu K, et al (2011) Advanced reproductive age and fertility. J Obstet Gynaecol Can 33: 1165–1175. Find this article online

  50. Puterman E, Lin J, Blackburn E, O’Donovan A, Adler N, et al. (2010) The power of exercise: buffering the effect of chronic stress on telomere length. PLoS One 5: e10837. Find this article online

  51. Parks CG, Miller DB, McCanlies EC, Cawthon RM, Andrew ME, et al. (2009) Telomere length, current perceived stress, and urinary stress hormones in women. Cancer Epidemiol Biomarkers Prev 8: 551–560. Find this article online

  52. Núñez MJ, Novío S, Amigo G, Freire-Garabal M (2011) The antioxidant potential of alprazolam on the redox status of peripheral blood leukocytes in restraint-stressed mice. Life Sci 89: 650–654. Find this article online

  53. Spitzer C, Gläser S, Grabe HJ, Ewert R, Barnow S, et al. (2011) Mental health problems, obstructive lung disease and lung function: findings from the general population. J Psychosom Res 71: 174–179. Find this article online

  54. Goldberg R, Morris P, Christian F, Badger J, Chabot S, et al. (1990) Panic disorder in cardiac outpatients. Psychosomatics 31: 168–173. Find this article online

  55. Li C, Barker L, Ford ES, Zhang X, Strine TW, et al. (2008) Diabetes and anxiety in US adults: findings from the 2006 Behavioral Risk Factor Surveillance System. Diabet Med 25: 878–881. Find this article online

  56. Savale L, Chaouat A, Bastuji-Garin S, Marcos E, Boyer L, et al. (2009) Shortened telomeres in circulating leukocytes of patients with chronic obstructive pulmonary disease. Am J Respir Crit Care Med 179: 566–571. Find this article online

  57. Epel ES (2009) Psychological and metabolic stress: a recipe for accelerated cellular aging? Hormones (Athens) 8: 7–22. Find this article online

  58. Lung FW, Chen NC, Shu BC (2007) Genetic pathway of major depressive disorder in shortening telomeric length. Psychiatr Genet 17: 195–199. Find this article online

  59. Hartmann N, Boehner M, Groenen F, Kalb R (2010) Telomere length of patients with major depression is shortened but independent from therapy and severity of the disease. Depress Anxiety 27: 1111–1116. Find this article online

  60. Kananen L, Surakka I, Pirkola S, Suvisaari J, Lönnqvist J, et al. (2010) Childhood adversities are associated with shorter telomere length at adult age both in individuals with an anxiety disorder and controls. PLoS One 5: e10826. Find this article online

  61. Surtees PG, Wainwright NW, Pooley KA, Luben RN, Khaw KT, et al. (2011) Life stress, emotional health, and mean telomere length in the European Prospective Investigation into Cancer (EPIC)-Norfolk population study. J Gerontol A Biol Sci Med Sci 66: 1152–1162. Find this article online

  62. American Psychiatric Association (1994) Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition. Washington, DC: APA.

  63. Keaney JF Jr, Larson MG, Vasan RS, Wilson PW, Lipinska I, et al. (2003) Obesity and systemic oxidative stress: clinical correlates of oxidative stress in the Framingham Study. Arterioscler Thromb Vasc Biol 23: 434–439. Find this article online

  64. Pou KM, Massaro JM, Hoffmann U, Vasan RS, Maurovich-Horvat P, et al. (2007) Visceral and subcutaneous adipose tissue volumes are cross-sectionally related to markers of inflammation and oxidative stress: the Framingham Heart Study. Circulation 116: 1234–1241. Find this article online

  65. Morrow JD, Frei B, Longmire AW, Gaziano JM, Lynch SM, et al. (1995) Increase in circulating products of lipid peroxidation (F2-isoprostanes) in smokers. Smoking as a cause of oxidative damage. N Engl J Med 332: 1198–1203. Find this article online

  66. Wang H, Yu M, Ochani M, Amella CA, Tanovic M, et al. (2003) Nicotinic acetylcholine receptor alpha7 subunit is an essential regulator of inflammation. Nature 421: 384–388. Find this article online

  67. van Dijk AP, Meijssen MA, Brouwer AJ, Hop WC, van Bergeijk JD, et al. (1998) Transdermal nicotine inhibits interleukin 2 synthesis by mononuclear cells derived from healthy volunteers. Eur J Clin Invest 28: 664–671. Find this article online

  68. Wyrobek AJ, Eskenazi B, Young S, Arnheim N, Tiemann-Boege I, et al. (2006) Advancing age has differential effects on DNA damage, chromatin integrity, gene mutations, and aneuploidies in sperm. Proc Natl Acad Sci U S A 103: 9601–9606. Find this article online

  69. Malaspina D (2001) Paternal factors and schizophrenia risk: de novo mutations and imprinting. Schizophr Bull 27: 379–393. Find this article online

  70. Frans EM, Sandin S, Reichenberg A, Lichtenstein P, Långström N, et al. (2008) Advancing paternal age and bipolar disorder. Arch Gen Psychiatry 65: 1034–1040. Find this article online

  71. Saha S, Barnett AG, Buka SL, McGrath JJ (2009) Maternal age and paternal age are associated with distinct childhood behavioural outcomes in a general population birth cohort. Schizophr Res 115: 130–135. Find this article online

  72. Irie M, Asami S, Nagata S, Ikeda M, Miyata M, et al. (2001) Psychosocial factors as a potential trigger of oxidative DNA damage in human leukocytes. Jpn J Cancer Res 92: 367–376. Find this article online

  73. Pitsavos C, Panagiotakos DB, Papageorgiou C, Tsetsekou E, Soldatos C, et al. (2006) Anxiety in relation to inflammation and coagulation markers, among healthy adults: the ATTICA study. Atherosclerosis 185: 320–326. Find this article online

  74. Drury SS, Theall K, Gleason MM, Smyke AT, De Vivo I, et al. (2011) Telomere length and early severe social deprivation: linking early adversity and cellular aging. Mol Psychiatry: [Epub ahead of print].

  75. Tyrka AR, Price LH, Kao HT, Porton B, Marsella SA, et al. (2010) Childhood maltreatment and telomere shortening: preliminary support for an effect of early stress on cellular aging. Biol Psychiatry 67: 531–534. Find this article online

  76. Hovatta I, Tennant RS, Helton R, Marr RA, Singer O, et al. (2005) Glyoxalase 1 and glutathione reductase 1 regulate anxiety in mice. Nature 438: 662–666. Find this article online

  77. Lecrubier Y (1998) The impact of comorbidity on the treatment of panic disorder. J Clin Psychiatry 59: 11–14; discussion 15–16.

  78. Kessler RC (2011) The National Comorbidity Survey (NCS) and its Extensions. In: Tsuang MT, Tohen M, Jones P, editors. pp. 221–241. pp.

  79. Hunt SC, Chen W, Gardner JP, Kimura M, Srinivasan SR, et al. (2008) Leukocyte telomeres are longer in African Americans than in whites: the National Heart, Lung, and Blood Institute Family Heart Study and the Bogalusa Heart Study. Aging Cell 7: 451–458. Find this article online

  80. Roux AV, Ranjit N, Jenny NS, Shea S, Cushman M, et al. (2009) Race/ethnicity and telomere length in the Multi-Ethnic Study of Atherosclerosis. Aging Cell 8: 251–257. Find this article online

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